We sequenced the genome and transcriptome of 3 male and 3 feminine folks from each one of the 4 target types Outcomes and Discussion (P. wingei, P. picta, Poecilia latipinna, and Gambusia holbrooki) (SI Appendix, Table S1) selected to express a even taxonomic distribution across Poeciliidae. For each species, we produced DNA sequencing (DNA-seq) with on average 222 million 150-base set (bp) paired-end reads (average insert size of 500 bp, leading to on average 76-fold protection) and 77.8 million 150-bp…